Transforming growth factor-β1-mediated collagen gel contraction by cardiac fibroblasts
نویسندگان
چکیده
Results TGF-β1 dose-dependently increased the contraction of collagen gel mediated by cardiac fibroblasts, reaching a maximal effect at 100 pmol/L TGF-β1. TGF-β1 also stimulated H-thymidine incorporation and total protein content in cardiac fibroblasts in the collagen gel lattice. TGF-β1 dose-dependently induced an increase in αsmooth muscle actin, a marker of myofibroblasts. The TGF-β1-induced reduction of area of the collagen gel was negatively correlated to the TGF-β1-evoked appearance of α-smooth muscle actin in the collagen gel matrix.
منابع مشابه
Transforming growth factor-beta 1-mediated collagen gel contraction by cardiac fibroblasts.
OBJECTIVE Myofibroblasts and transforming growth factor-beta(1) (TGF-beta(1)) are key elements of cardiac tissue fibrosis development. The aim of this study was to determine whether the ability of TGF-beta(1) to affect the contractile activity of cardiac fibroblasts depends on their differentiation into myofibroblasts. METHODS Cardiac fibroblasts (from male adult Wistar rats) from passage two...
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